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miniprotean ii multiscreen apparatus  (Bio-Rad)


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    Structured Review

    Bio-Rad miniprotean ii multiscreen apparatus
    HEV-C1-P241 immunoblot of rHEV EIA-positive companion animal sera samples. (A) Immunoblot of rHEV EIA-positive dog sera. (B) Immunoblot of rHEV EIA-positive cat sera. N: blank lane; P: anti-His antibody control. Blot assays were performed in a <t>MiniProtean</t> II Apparatus, which enables lane-by-lane separation of the immunoblot without cutting into strips.
    Miniprotean Ii Multiscreen Apparatus, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/miniprotean+ii+multiscreen+apparatus/mini+protean+tgx+precast+protein+gels/pmc11018080-84-20-24
    Average 90 stars, based on 1 article reviews
    miniprotean ii multiscreen apparatus - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Rat hepatitis E virus (Rocahepevirus ratti) exposure in cats and dogs, Hong Kong"

    Article Title: Rat hepatitis E virus (Rocahepevirus ratti) exposure in cats and dogs, Hong Kong

    Journal: Emerging Microbes & Infections

    doi: 10.1080/22221751.2024.2337671

    HEV-C1-P241 immunoblot of rHEV EIA-positive companion animal sera samples. (A) Immunoblot of rHEV EIA-positive dog sera. (B) Immunoblot of rHEV EIA-positive cat sera. N: blank lane; P: anti-His antibody control. Blot assays were performed in a MiniProtean II Apparatus, which enables lane-by-lane separation of the immunoblot without cutting into strips.
    Figure Legend Snippet: HEV-C1-P241 immunoblot of rHEV EIA-positive companion animal sera samples. (A) Immunoblot of rHEV EIA-positive dog sera. (B) Immunoblot of rHEV EIA-positive cat sera. N: blank lane; P: anti-His antibody control. Blot assays were performed in a MiniProtean II Apparatus, which enables lane-by-lane separation of the immunoblot without cutting into strips.

    Techniques Used: Western Blot

    Related Articles

    other:

    Article Title: Lhca5 interaction with plant photosystem I.
    Article Snippet: In the outer antenna (LHCI) of higher plant photosystem I (PSI) four abundantly expressed light-harvesting protein of photosystem I (Lhca)-type proteins are organized in two heterodimeric domains (Lhca1/Lhca4 and Lhca2/Lhca3).. Our cross-linking studies on PSI-LHCI preparations from wildtype Arabidopsis and pea plants indicate an exclusive interaction of the rarely expressed Lhca5 light-harvesting protein with LHCI in the Lhca2/Lhca3-site.. In PSI particles with an altered LHCI composition Lhca5 assembles in the Lhca1/Lhca4 site, partly as a homodimer.

    Article Title: Generation and use of polyclonal and monoclonal antibodies specific for 3-phosphohistidine
    Article Snippet: IgG concentrations of hybridoma cell-supernatants (obtained from IgG ELISA assays performed by Epitomics) were normalized to 0.5 ug/ml for screening of anti-pHis mAbs (dilution factors ranged from 1:5 to 1:500) by dilution with casein blocking buffer supplemented with 0.1% Tween-20.

    Article Title: Global profiling of the signaling network of papillary thyroid carcinoma.
    Article Snippet: Aims: Thyroid carcinoma is one of the most fast rising cancer diagnoses in US.. Papillary thyroid carcinoma (PTC) comprises 80% of thyroid carcinoma.. The goal of our study is to identify regulatory proteins and signaling pathways altered in PTC.

    Article Title: Co-expression of XIAP and cyclin D1 complex correlates with a poor prognosis in patients with hepatocellular carcinoma.
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    Incubation:

    Article Title: Rat hepatitis E virus (Rocahepevirus ratti) exposure in cats and dogs, Hong Kong
    Article Snippet: .. Sera of dogs and cats were diluted 1:200 with 10% skim milk in PBS-T and added to membranes in the MiniProtean II Multiscreen Apparatus (BioRad, Hercules, USA) and incubated for one hour. ..

    Article Title: Production of outer surface protein A byBorrelia burgdorferiduring transmission from infected mammals to feeding ticks is insufficient to trigger OspA seroconversion
    Article Snippet: .. Membranes were washed after blocking, then simultaneously incubated for 1 h at room temperature with each mouse serum sample, diluted 1 : 100 in TBS–T, using a MiniProtean II Multiscreen apparatus (Bio-Rad, Hercules, CA). ..

    Article Title: Anti-retinal IgG antibodies in patients with early and advanced type 2 macular telangiectasia.
    Article Snippet: Proteins were separated by gel electrophoresis in MES buffer (Invitrogen), and then transferred onto a PVDF membrane (Immobilon-FL, Merck Millipore, Billerica, MA, USA) at 80V for 90 min in transfer buffer (25 mM Tris, 190 mM glycine, 20% methanol, pH 8.3). .. After transfer, the membrane was incubated with blocking buffer (5% BSA in TBS) at room temperature for 1 h. Membranes were then loaded onto MiniPROTEAN® II Multiscreen Apparatus (Biorad) and incubated with patient serum (1:200) or rabbit anti-RP1 antibody (1:1000, HPA042257, S. McLenachan et al. .. Experimental Eye Research 218 (2022) 109024 Sigma) diluted in blocking buffer at 4 ◦C overnight.

    Blocking Assay:

    Article Title: Production of outer surface protein A byBorrelia burgdorferiduring transmission from infected mammals to feeding ticks is insufficient to trigger OspA seroconversion
    Article Snippet: .. Membranes were washed after blocking, then simultaneously incubated for 1 h at room temperature with each mouse serum sample, diluted 1 : 100 in TBS–T, using a MiniProtean II Multiscreen apparatus (Bio-Rad, Hercules, CA). ..

    Article Title: Anti-retinal IgG antibodies in patients with early and advanced type 2 macular telangiectasia.
    Article Snippet: Proteins were separated by gel electrophoresis in MES buffer (Invitrogen), and then transferred onto a PVDF membrane (Immobilon-FL, Merck Millipore, Billerica, MA, USA) at 80V for 90 min in transfer buffer (25 mM Tris, 190 mM glycine, 20% methanol, pH 8.3). .. After transfer, the membrane was incubated with blocking buffer (5% BSA in TBS) at room temperature for 1 h. Membranes were then loaded onto MiniPROTEAN® II Multiscreen Apparatus (Biorad) and incubated with patient serum (1:200) or rabbit anti-RP1 antibody (1:1000, HPA042257, S. McLenachan et al. .. Experimental Eye Research 218 (2022) 109024 Sigma) diluted in blocking buffer at 4 ◦C overnight.

    Membrane:

    Article Title: Anti-retinal IgG antibodies in patients with early and advanced type 2 macular telangiectasia.
    Article Snippet: Proteins were separated by gel electrophoresis in MES buffer (Invitrogen), and then transferred onto a PVDF membrane (Immobilon-FL, Merck Millipore, Billerica, MA, USA) at 80V for 90 min in transfer buffer (25 mM Tris, 190 mM glycine, 20% methanol, pH 8.3). .. After transfer, the membrane was incubated with blocking buffer (5% BSA in TBS) at room temperature for 1 h. Membranes were then loaded onto MiniPROTEAN® II Multiscreen Apparatus (Biorad) and incubated with patient serum (1:200) or rabbit anti-RP1 antibody (1:1000, HPA042257, S. McLenachan et al. .. Experimental Eye Research 218 (2022) 109024 Sigma) diluted in blocking buffer at 4 ◦C overnight.

    Article Title: Improved pharmacokinetics and immunogenicity profile of organophosphorus hydrolase by chemical modification with polyethylene glycol.
    Article Snippet: Contents lists available at ScienceDirect Journal of Controlled Release j ourna l homepage: www.e lsev ie r.com/ locate / jconre l Improved pharmacokinetics and immunogenicity profile of organophosphorus hydrolase by chemical modification with polyethylene glycol Boris N. Novikov, Janet K. Grimsley, Rory J. Kern, James R. Wild, Melinda E. Wales ⁎ Department of Biochemistry and Biophysics, 2128 Texas A&M University, College Station, TX 77843-2128, USA ⁎ Corresponding author.. Tel.. : +1 979 845 9459; fax: E-mail address: m-wales@tamu.edu (M.E.

    Clinical Proteomics:

    Article Title: Improved pharmacokinetics and immunogenicity profile of organophosphorus hydrolase by chemical modification with polyethylene glycol.
    Article Snippet: Contents lists available at ScienceDirect Journal of Controlled Release j ourna l homepage: www.e lsev ie r.com/ locate / jconre l Improved pharmacokinetics and immunogenicity profile of organophosphorus hydrolase by chemical modification with polyethylene glycol Boris N. Novikov, Janet K. Grimsley, Rory J. Kern, James R. Wild, Melinda E. Wales ⁎ Department of Biochemistry and Biophysics, 2128 Texas A&M University, College Station, TX 77843-2128, USA ⁎ Corresponding author.. Tel.. : +1 979 845 9459; fax: E-mail address: m-wales@tamu.edu (M.E.

    Western Blot:

    Article Title: Improved pharmacokinetics and immunogenicity profile of organophosphorus hydrolase by chemical modification with polyethylene glycol.
    Article Snippet: Contents lists available at ScienceDirect Journal of Controlled Release j ourna l homepage: www.e lsev ie r.com/ locate / jconre l Improved pharmacokinetics and immunogenicity profile of organophosphorus hydrolase by chemical modification with polyethylene glycol Boris N. Novikov, Janet K. Grimsley, Rory J. Kern, James R. Wild, Melinda E. Wales ⁎ Department of Biochemistry and Biophysics, 2128 Texas A&M University, College Station, TX 77843-2128, USA ⁎ Corresponding author.. Tel.. : +1 979 845 9459; fax: E-mail address: m-wales@tamu.edu (M.E.



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    HEV-C1-P241 immunoblot of rHEV EIA-positive companion animal sera samples. (A) Immunoblot of rHEV EIA-positive dog sera. (B) Immunoblot of rHEV EIA-positive cat sera. N: blank lane; P: anti-His antibody control. Blot assays were performed in a <t>MiniProtean</t> II Apparatus, which enables lane-by-lane separation of the immunoblot without cutting into strips.
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    HEV-C1-P241 immunoblot of rHEV EIA-positive companion animal sera samples. (A) Immunoblot of rHEV EIA-positive dog sera. (B) Immunoblot of rHEV EIA-positive cat sera. N: blank lane; P: anti-His antibody control. Blot assays were performed in a <t>MiniProtean</t> II Apparatus, which enables lane-by-lane separation of the immunoblot without cutting into strips.
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    Image Search Results


    HEV-C1-P241 immunoblot of rHEV EIA-positive companion animal sera samples. (A) Immunoblot of rHEV EIA-positive dog sera. (B) Immunoblot of rHEV EIA-positive cat sera. N: blank lane; P: anti-His antibody control. Blot assays were performed in a MiniProtean II Apparatus, which enables lane-by-lane separation of the immunoblot without cutting into strips.

    Journal: Emerging Microbes & Infections

    Article Title: Rat hepatitis E virus (Rocahepevirus ratti) exposure in cats and dogs, Hong Kong

    doi: 10.1080/22221751.2024.2337671

    Figure Lengend Snippet: HEV-C1-P241 immunoblot of rHEV EIA-positive companion animal sera samples. (A) Immunoblot of rHEV EIA-positive dog sera. (B) Immunoblot of rHEV EIA-positive cat sera. N: blank lane; P: anti-His antibody control. Blot assays were performed in a MiniProtean II Apparatus, which enables lane-by-lane separation of the immunoblot without cutting into strips.

    Article Snippet: Sera of dogs and cats were diluted 1:200 with 10% skim milk in PBS-T and added to membranes in the MiniProtean II Multiscreen Apparatus (BioRad, Hercules, USA) and incubated for one hour.

    Techniques: Western Blot